The Royal Children’s Hospital of Melbourne has a great clinical guideline for pediatric lumbar puncture

22 gauge spinal needle can be used in all age groups. Use 1.5 inch length in < 2-3 year olds, 3.5 inch length in older (can use 2.5 inch length in 2-12 year olds if available)

20 drops of CSF = ~ 1 mL

AgeWeight (kg)MSCD (cm)Needle length cmNeedle length in
Neonate / Infant1-50.9-1.7cm20.8
Toddler 12-18mo102.4cm31.2
4yo152.6cm3-41.2-1.6
6yo202.8cm41.6
9yo303.2cm41.6
12yo403.6cm52
14yo504cm5-62-2.4

Formulas to estimate median spinal cord depth (MCSD, mm):

Neonates and infants: 2 (wt in kg) + 7 mm

Children: 0.4 (wt in kg) + 20 mm